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村井 耕二むらい こうじ

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所属 生物資源学部 / 創造農学科生物資源学研究科
職名 教授
研究室 あわらキャンパス 研究棟22室 (内線 0776-68-8322)
E-mail murai (末尾に「@fpu.ac.jp」をつけてください)
ウェブサイト komugi-koji
最終学歴 京都大学大学院農学研究科修士課程修了
学位 京都大学博士 (農学) 1992.7
所属学会 日本育種学会
日本遺伝学会
日本植物生理学会
日本分子生物学会
日本作物学会
職歴 1986. 4 住友化学工業 株式会社 研究員
1993. 4 石川県農業短期大学助教授
1998.10 福井県立大学助教授
2004. 4 現職

研究活動

専門 植物遺伝育種学・植物発生遺伝学
研究活動の紹介 1.早生多収コムギ品種育成のための基礎研究
コムギが葉を作る栄養成長期から穂を作る生殖成長期へ移行する際、どのような遺伝子がどのように働いているのか、また、穂の形がどのような遺伝子の働きによって決定されるのか、さらに、雄しべ雌しべなどの花の器官がどのような遺伝子の働きによって作られるのか、を研究しています。そして、これらの知見を利用して、福井県の栽培に適した早生多収コムギ品種の開発を行なっています。
2.雑草防除用リビングマルチコムギ開発のための基礎研究
大豆、夏野菜、果樹園などの雑草防除用のリビングマルチコムギの開発を行っています。
3.パスタ用デュラムコムギ品種育成のための基礎研究
国内初のパスタ用のコムギ品種育成に向け、研究を開始しました。
キーワード 遺伝学 DNA 遺伝子 染色体 ゲノム 発生 遺伝子組換え 品種改良 育種 小麦

著書・論文

レフリー付き原著論文 業績リスト 村井耕二

Matsuta. A, T. Mayuzumi, H. Katano, M. Hatashita, K. Takagi, Y. Hayashi, T. Abe, K. Murai and Y. Kazama (2021) The effect of heavy-ion beams with high linear energy transfer on mutant production in M1 generation of Torenia fournieri. Cytologia 86(4): 317–322. DOI: 10.1508/cytologia.86.317

Hashimoto. K, Y. Kazama, H. Ichida, T. Abe and K. Murai* (2021) Einkorn wheat (Triticum monococcum) mutant extra-early flowering 4, generated by heavy-ion beam irradiation, has a deletion of the LIGHT-REGULATED WD1 homolog. Cytologia 86(4): 297–302. DOI: 10.1508/cytologia.86.297

Itoh, T*, A. Kamiya, M. Kimura and K. Murai* (2021) Heterogeneous Expression and Purification of the Wheat VRN1 K-Box Domain Suggest the Formation of a Tetramer of the VRN1 Protein. American J. Plant Sci. 12: 1002-1010. https://doi.org/10.4236/ajps.2021.127068

Titan, P., J. Iskra, K. Murai and V. Meglič (2020) Chemically induced male sterility in common wheat mediated by Aegilops cytoplasm. Genetica 52: 245-256.
https://doi.org/10.2298/GENSR2001245T

Takumi, S*, Seito Mitta, S. Komura, T. M. Ikeda, H. Matsunaka, K. Sato, K. Yoshida, K. Murai* (2020) Introgression of chromosomal segments conferring early heading date from wheat diploid progenitor, Aegilops tauschii Coss., into Japanese elite wheat cultivars. PLOS ONE | https://doi.org/10.1371/journal.pone.0228397

Murai, K., H. Ohta, Y. Takenouchi, M. Kurushima, N. Ishikawa, V. Meglič and P. Titan (2019) Trials for hybrid seed production and estimation of wheat F1 hybrids produced by outcrossing using photoperiod-sensitive cytoplasmic male sterile (PCMS) system with elite lines. J. Agr. Crop Res. 7: 119-126.
Nishiura, A., S. Kitagawa, M. Matsumura, Y. Kazama, T. Abe, N. Mizuno, S. Nasuda and K. Murai (2018) An early-flowering einkorn wheat mutant with deletions of PHYTOCLOCK 1/LUX ARRHYTHMO and VERNALIZATION 2 exhibits a high level of VERNALIZATION 1 expression induced by vernalization. J. Plant Physiol. 222: 28-38.

Miura, S., N. Crofts, M. Abe, K. Murai, K. Iwaki, S. Fujita and N. Fujita (2018) Identification of a point mutation in the Granule-bound starch synthase (GBSSI) gene in a waxy diploid wheat mutant and design of molecular markers for backcrossing. J. Applied Glycoscience 65:9-11.

Tanaka, C., T. Itoh, Y. Iwasaki, N. Mizuno, S. Nasuda and K. Murai (2018) Direct interaction between VRN1 protein and the promoter region of the wheat FT gene. Genes Genet. Syst. 93: 25-29.

Mizuno, N., M. Kinoshita, S. Kinoshita, H. Nishida, M. Fujita, K. Kato, K. Murai and S. Nasuda (2016) Non-functional mutations in three homoeologous PHYTOCLOCK 1 genes in common wheat are associated with the extra-early flowering phenotype. PLoS ONE 11(10), e0165618

Murai, K., H. Ohta, M. Kurushima and N. Ishikawa (2016) Photoperiod-sensitive cytoplasmic male sterile elite lines for hybrid wheat breeding, showing high cross-pollination fertility under long-day conditions. Euphytica 212: 313-322.

Tanaka, M., H. Tanaka, N. Shitsukawa, S. Kitagawa, S. Takumi and K. Murai (2015) Homoeologous copy-specific expression patterns of MADS-box genes for floral formation in allopolyploid wheat. Genes Genet. Syst. 90: 217-229.

Nguyen, A. T., R. Nishijima, T. Kajimura, K. Murai and S. Takumi (2015) Quantitative trait locus analysis for flowering-relatedtraits using two F2 populations derived from crosses between Japanese common wheat cultivarsand synthetic hexaploids. Genes Genet. Syst. 90: 89-98.

Nishiura, A., Y. Kazama, T. Abe, N. Mizuno, S. Nasuda and K. Murai (2014) Level of VERNALIZATION 1 expression is correlated with earliness in extra early-flowering mutant wheat lines. Breed. Science 64: 213-221.

Tahira, C., N. Shitsukawa, Y. Kazama, T. Abe and K. Murai (2013) The wheat plastochron mutant, fushi-darake, shows transformation of reproductive spikelet meristm into vegetative shoot meristem. American J. Plant Sci. 4: 28-36.

Murai, K., A. Nishiura, Y. Kazama and T. Abe (2013) A large-scale mutant panel in wheat developed using heavy-ion beam mutagenesis and its application to genetic research. Nuclear Instruments and Methods in Physics Research B: Beam Interactions with Materials and Atoms 314: 59-62. http://dx.doi.org/10.1016/j.nimb.2013.5.026

Murai, K. (2013) Homeotic genes and the ABCDE model for floral organ formation in wheat. Plants 2: 379-395.

Nguyen, A. T., J. C. M. Iehisa, T. Kajimura, K. Murai and S. Takumi (2013) Identification of quantitative trait loci for flowering-related traits in the D genome of synthetic hexaploid wheat lines. Euphytica 192: 401-412.

Yamamoto, M., N. Shitsukawa, M. Yamada, K. Kato, S. Takumi, K. Kawaura, Y. Ogihara and K. Murai (2012) Identification of a novel homolog for a calmodulin-binding protein that is upregulated in alloplasmic wheat showing pistillody. Planta 237: 1001-1013.

Kitagawa, S., S. Shimada and K. Murai (2012) Effect of Ppd-1 on the expression of flowering-time genes in vegetative and reproductive growth stages of wheat. Genes Genet. Syst. 87: 161-168.

Kinjo, H., N. Shitsukawa, S. Takumi and K. Murai (2012) Diversification of three APETALA1/FRUITFULL-like genes in wheat. Mol. Genet. Genomics 287: 283-294.

Kajimura, T., K. Murai and S. Takumi (2011) Distinct genetic regulation of lowering time and grain-filling period base on empirical study of D-genome diversity in synthetic hexaploid wheat lines. Breed. Sci. 61: 130-141.

Fujiwara, Y., S. Shimada, S. Takumi and K. Murai (2010) Differential effects of Aegilops tauschii genotypes on maturing-time in synthetic hexaploid wheats. Breed. Sci. 60(3): 286-292.

Yamagishi, M., Y. Takeuchi, I. Tanaka, I. Kono, K. Murai and M. Yano (2010) Segregation distortion in F2 and doubled haploid populations of temperate japonica rice. J. Genetics 89(2): 237-241.

Omori, H., M. Hosokawa, H. Shiba, N. Shitsukawa, K. Murai and S. Yazawa (2009) Screening of Chrysanthemum plants with strong resistance to Chrysanthemum Stunt Viroid. J. Japan. Soc. Hort. Sci. 78(3): 350-355.

Yamada, K., T. Saraike, N. Shitsukawa, C. Hirabayashi, S. Takumi and K. Murai (2009) Class D and Bsister MADS-box genes are associated with ectopic ovule formation in the pistil-like stamens of alloplasmic wheat (Triticum aestivum L.). Plant Mol. Biol. 71: 1-14.

Ishikawa, M., Y. Ohmori, W. Tanaka, C. Hirabayashi, K. Murai, Y. Ogihara, T. Yamaguchi and H.-Y. Hirano (2009) The spatial expression patterns of DROOPING LEAF orthologs suggest a conserved function in grasses. Genes Genet. Syst. 84: 137-146.

Shitsukawa, N., H. Kinjo、S. Takumi and K. Murai (2009) Heterochronic development of the floret meristem determines grain number per spikelet in diploid, tetraploid and hexaploid wheats. Annals of Botany 104(2): 243-251.

Shimada, S., T. Ogawa, S. Kitagawa, T. Suzuki, C. Ikari, N. Shitsukawa, T. Abe, H. Kawahigashi, R. Kikuchi, H. Handa and K. Murai (2009) A genetic network of flowering time genes in wheat leaves, in which an APETALA1/FRUITFULL-like gene, VRN1, is upstream of FLOWERING LOCUS T. Plant J. 58: 668-681.

Mizumoto, K., H. Hatano, C. Hirabayashi, K. Murai and S. Takumi (2009) Altered expression of wheat AINTEGUMENTA homolog, WANT-1, in pistil and pistil-like transformed stamen of an alloplasmic line with Aegilops crassa cytoplasm. Dev. Genes Evol. 219: 175-187.

Morimoto, R., E, Nishioka, K. Murai and S. Takumi (2009) Functional conservation of wheat orthologs of maize rough sheath1 and Rough Sheath2 genes. Plant Mol. Biol. 69: 273-285.

Zhu, Y., T. Saraike, Y. Yamamoto, H. Hagita, S. Takumi and K. Murai (2008) orf260cra, a novel mitochondrial gene, is associated with the homeotic transformation of stamens into pistil-like structures (pistillody) in alloplasmic wheat. Plant Cell Phsyol. 49: 1723-1733.

Aida, R., M. Komano, M. Saito, K. Nakase and K. Murai (2008) Chrysanthemum flower shape modification by suppression of chrysanthemum-AGAMOUS gene. Plant Biotechnology 25: 55-59.

Murai, K., I. Tsutui, Y. Kawanishi, S. Ikeguchi, M. Yanaka and N. Ishikawa (2008) Development of photoperiod-sensitive cytoplasmic male sterile (PCMS) wheat lines showing high male sterility under long-day conditions and high seed fertility under short-day conditions. Euphytica 159: 315-323.

Saraike, T., N. Shitsukawa, Y. Yamamoto, H. Hagita, Y. Iwasaki, S. Takumi, and K. Murai (2007) Identification of a protein kinase gene associated with pistillody, homeotic transformation of stamens into pistil-like structures, in alloplasmic wheat. Planta 227: 211-221.

Ishibashi, M., F. Kobayashi, J. Nakamura, K. Murai and S. Takumi (2007) Variation of freezing tolerance, Cor/Lea gene expression and vernalization requirement in Japanese common wheat. Plant Breed. 126: 464-469.

Shitsukawa, N., C. Tahira, K.-I. Kassai, C. Hirabayashi, T. Shimizu, S. Takumi, K. Mochida, K. Kwaura, Y. Ogihara and K. Murai (2007) Genetic and epigenetic alteration among three homoeologous genes of a class E MADS box gene in hexaploid wheat. Plant Cell 19: 1723-1737.

Sugie, A., K. Murai and S. Takumi (2007) Alteration of respiration capacity and expression level of alternative oxidase genes in necrosis lines of common wheat. Genes Genet. Syst. 82: 231-239.

Shitsukawa, N., C. Ikari, T. Mitsuya, T. Sakiyama, A. Ishikawa, S. Takumi and K. Murai (2007) Wheat SOC1 functions independently o WAP1/VRN1, an integrator of vernalization and photoperiod flowering promotion pathways. Physiologia Plantarum 130: 627-636.

Shitsukawa, N., C. Ikari, S. Shimada, S. Kitagawa, K. Sakamoto, H. Saito, H. Ryuto, N. Fukunishi, T. Abe, S. Takumi, S. Nasuda and K. Murai (2007) The einkorn wheat (Triticum monococcum) mutant, maintained vegetative phase, is caused by a deletion in the VRN1 gene. Genes Genet. Syst. 82: 167-170.

Shitsukawa, N., A. Takagishi, C. Ikari, S. Takumi and K. Murai (2006) WFL, a wheat FLORICAULA/LEAFY ortholog, is associated with spikelet formation as lateral branch of the inflorescence meristem. Genes Genet. Syst. 81: 13-20.

Ogihara, Y., Y. Yamazaki, K. Murai, A. Kanno, T. Terachi, T. Shiina, N. Miyashita, S. Nashuda, C. Nakamura, N. Mori, S. Takumi, M. Murata, S. Futo and K. Tsunewaki (2005) Structural dynamics of cereal mitochondrial genomes as revealed by complete uncleotide sequencing of the wheat mitochondrial genome. Nucleic Acids Research 33: 6235-6250.

Kobayashi, F., S. Takumi, S. Kume, M. Ishibashi, R. Ohno, K. Murai and C. Nakamura (2005) Regulation by Vrn-1/Fr-1 chromosomal intervals of CBF-mediated Cor/Lea gene expression and freezing tolerance in common wheat. J. Exp. Botany 56: 887-895.

Taketa, S., T. Awayama, M. Ichii, M. Sunakawa, T. Kawahara and K. Murai (2005) Molecular cytogenetic identification of nullisomy 5B induced homoeologous recombination between wheat chromosome 5D and barley chromosome 5H. Genome 48: 115-124.

Ogihara, Y., K. Mochida, K. Kawaura, K. Murai, M. Seki, A. Kamiya, S. Shinozaki, P. Carninci, Y. Hayashizaki, T. Shin-I, Y. Kohara and Y. Yamazaki (2004) Construction of a full-length cDNA library from young spikelets of hexaploid wheat and its characterization by large-scale sequencing of expressed sequence tags. Genes Genet. Syst. 79: 227-232.

Mizumoto, K., K. Murai, C. Nakamura and S. Takumi (2004) Preferential expression of a HLP homolog encoding a mitochondrial L14 rebosomal protein in stamens of common wheat. Gene 343: 281-289.

Hama, E., S. Takumi, Y. Ogihara and K. Murai (2004) Pistillody is caused by alterations of the class B MADS box gene expression pattern in alloplasmic wheats. Planta 218: 712-720.

Murai, K., M. Miyamae, H. Kato, S. Takumi and Y. Ogihara (2003) WAP1, a wheat APETALA1 homolog, plays a central role in the phase transition from vegetative to reproductive growth. Plant Cell Physiol. 44: 1255-1265.

Ogihara, Y., K. Mochida, Y. Nemoto, K. Murai, Y. Yamazaki, T. Shin-i, Y. Kohara (2003) Correlated clustering and virtual display of gene expression patterns in the wheat life cycle by large-scale statistical analyses of expressed sequence tags. Plant J. 33: 1001-1011.

Meguro, A., S. Takumi, Y. Ogihara and K. Murai (2003) WAG, a wheat AGAMOUS homolog, is associated with development of pistil-like stamens in alloplasmic wheats. Sex. Plant Reprod. 15: 221-230.

Kawahara, T., S. Taketa and K. Murai (2002) Differential effects of cultivated and wild barley 5H chromosomes on heading characters in wheat-barley chromosome addition lines. Hereditas 136: 195-200.

Murai, K. (2002) Comparison of two fertility restoration systems against photoperiod-sensitive cytoplasmic male sterility in wheat. Plant Breed. 121: 363-365.

Tsunewaki, K., K. Murai, S. Ohta and G.ミZ. Wang (2002) Plasmon analysis provides a critical clue to correct classification of a plant taxon ミ A case of the goat grass genus, Aegilops. Cytologia 67: 95-104.

Murai, K., S. Takumi, H. Koga and Y. Ogihara (2002) Pistillody, homeotic transformation of stamens into pistil-like structures, caused by nuclear-cytoplasm interaction in wheat. Plant J. 29: 169-181.

Ogihara, Y., K. Isono, T. Kojima, A. Endo, M. Hanaoka, T. Shiina, T. Terachi, S. Utsugi, M. Murata, N. Mori, S. Takumi, K. Ikeo, T. Gojobori, R. Murai, K. Murai, Y. Matsuoka, Y. Ohnishi, H. Tajiri and K. Tsunewaki (2002) Structural features of a wheat plastome as revealed by complete sequencing of chloroplast DNA. Mol. Genet. Genomics 266: 740-746.

Kato, H., S. Taketa, T. Ban, N. Iriki and K. Murai (2001) The influence of a spring habit gene, Vrn-D1, on heading time in wheat. Plant Breed. 120: 115-120.

Murai, K. (2001) Factors responsible for levels of male sterility in photoperiod-sensitive cytoplasmic male sterile (PCMS) wheat lines. Euphytica 117:111-116.

Murai, K., S. Taketa, A. K. M. R. Islam and K. W. Shepherd (2000) Barley allele-specific amplicons useful for identifying wheat-barley recombinant chromosomes. Genes Genet. Syst. 75:131-139.

Takumi, S., T. Kosugi, K. Murai, N. Mori and C. Nakamura (2000) Molecular cloning of three homoeologous cDNAs encoding orthologs of the maize KNOTTED1 homeobox protein from young spikes of hexaploid wheat. Gene 249:171-181.

Ogihara, Y., Y. Kurihara, K. Futami and K. Murai (1999) Photoperiod-sensitive cytoplasmic male sterility in wheat: nuclear-mitochondrial incompatibility results in differential processing of the mitichondrial orf25 gene. Curr. Genet. 36:354-362.

Takumi, S., K. Murai, N. Mori and C. Nakamura (1999) Variations in the maize Ac trsnsposase transcript level and the Ds excision frequency in transgenic wheat callus lines. Genome 42:1234-1241.

Takumi, S., K. Murai, N. Mori and C. Nakamura (1999) Trans-activation of a maize Ds transposable element in transgenic wheat plants expressing the Ac transposase gene. Theor. Appl. Genet. 98:947-953.

Murai, K. (1998) F1 seed production efficiency by using photoperiod-sensitive cytoplasmic male sterility and performance of F1 hybrid lines in wheat. Breed. Sci. 48:35分-40.

Ogihara, Y., Y. Aizawa, N. Kawakami and K. Murai (1998) Cloning of cDNAs specifically expressed in wheat spikelets at the heading stage, as identified by the simple differential display method. Plant Sci. 135:49-62.

Murai, K. (1997) Effects of Aegilops crassa cytoplasm on the agronomic characters in photoperiod-sensitive CMS wheat lines and F1 hybrids. Breed. Sci. 47:321-326.

Murai, K. (1997) Genetic analysis of fertility restoration against photoperiod-sensitive cytoplasmic male sterility in Triticum aestivum cv. Norin 61. Plant Breed. 116:592-594.

Murai, K., T. Koba and T. Shimada (1997) Effects of barley chromosome on heading characters in wheat-barley chromosome addition lines. Euphytica 96:281-287.

Murai, K., R. Murai and Y. Ogihara (1997) Wheat MADS box genes, a multigene family dispersed throughout the genome. Genes Genet. Syst. 72:317-321.

Ogihara, Y., K. Futami, T. Tsuji and K. Murai (1997) Alloplasmic wheats with Aegilops crassa cytoplasm which express photoperiod-sensitive homeotic transformations of anthers, show alterations in mitochondrial DNA structure and transcription. Mol. Gen. Genet. 255:45-53.

Nakamura, M., J. Miyamoto, K. Murai, M. Yamagishi and K. Fukui (1997) The rDNA locus and the RAPDs analysis of Solidago virgaurea ssp. asiatica and
ssp. leiocarpa (Compositae) in Mt. Hakusan. J. Phytogeography Taxon.

Murai, R. and K. Murai (1996) Different transcriptional regulation of cytosolic and plastidic Cu/Zn-superoxide dismutase genes in Solidago altissima (Asteraceae). Plant Sci. 120:71-79.

Murai, K. and K. Tsunewaki (1995) Photoperiod-sensitive cytoplasmic male sterility induced in Japanese wheat cultivars by transferring Aegilops crassa cytoplasm. Breed. Sci. 45:199-203.

Murai, K., Y. Ogihara and K. Tsunewaki (1995) An EMS-induced wheat mutant restoring fertility against photoperiod-sensitive cytoplasmic male sterility. Plant Breed. 114:205-209.

Abo-elwafa, A., K. Murai and T. Shimada (1995) Intra- and inter-specific variations in Lens revealed by RAPD markers. Theor. Appl. Genet. 90:335-340.

Murai, K. and K. Tsunewaki (1994) Genetic analysis on the fertility restoration by Triticum aestivum cv. Chinese Spring against photoperiod-sensitive cytoplasmic male sterility. Jpn. J. Genet. 69:195-202.

Atanassov, Z., C. Nakamura, N. Mori, C. Kaneda, H. Kato, Y.-Z. Jin, T. Yoshizawa and K. Murai (1994) Mycotoxin production and pathogenicity of Fusarium species and wheat resistance to Fusarium head blight. Can. J. Bot. 72:161-167.

Murai, K. and K. Tsunewaki (1993) Photoperiod-sensitive cytoplasmic male sterility in wheat with Aegilops crassa cytoplasm. Euphytica 67:41-48.

Murai, K., N. Xu and K. Tsunewaki (1989) Studies on the origin of crop species by restriction endonuclease analysis of organellar DNA. III. Chloroplast DNA variation and interspecific relationships in the genus Secale. Jpn. J. Genet. 64時35分-47.

Murai, K. and K. Tsunewaki (1987) Chloroplast genome evolution in the genus Avena. Genetics 116:613-621.

Murai, K. and K. Tsunewaki (1986) Molecular basis of genetic diversity among cytoplasms of Triticum and Aegilops species. VI. CtDNA variation in Ae. triuncialis. Heredity 57:335-339.

社会・地域活動

日本育種学会幹事(平成14から17年度、平成20から23年度、平成26年度から) 日本育種学会常任幹事(平成16から17年度) 日本遺伝学会学会誌(Genes & Genetic Systems)編集委員(平成18年度から) 日本作物学会北陸支部・北陸育種談話会支部長(平成16年7月から18年7月) 放送大学非常勤講師(平成17年度から18年度、平成25年度) イオンビーム育種研究会幹事(平成20年度から) 福井県立大学オープンカレッジ講師(毎年開催) 松岡中学校地域・学校協議会委員(平成20から21年度) 大学入試センター試験出題委員(生物)(平成21年度から22年度) 文部科学省各種評価委員(平成21年度から) 福井大学未来の科学者養成講座評価委員(平成21年度から)日本学術会議連会員(平成23年10月から)

教育活動

担当科目 育種学Ⅰ、実践農業英語、その他(学部)、植物発生遺伝学特論(大学院)
オフィスアワー Lunch time hour on every day (12時10分-13時)
相談・講演・共同研究等に応じられるテーマなど ・暮しの中の遺伝学 (酒豪の遺伝子、血液型と性格、など) ・DNA-遺伝子-染色体-ゲノム (遺伝子、ゲノムなんでも相談応じます) ・発生生物学 (動植物の体はどのように作られるか?万能細胞とは?) ・遺伝子組換え植物 (本当は安全なの?危険なの?どうやって作るの?) ・植物の品種改良 (コシヒカリはこのようにして生まれた、など) ・福井県小麦特産化プロジェクト!

その他

受賞:平成10年日本育種学会奨励賞 1999.4
平成17年日本作物学会貢献賞 2006.3
平成22年日本遺伝学会論文賞 2010.9
コメント:植物遺伝育種学/植物発生遺伝学を中心に分子生物学、 植物生理学との境界領域を指向しています。
趣味:ギター弾き語り、 バンド、 魚釣り (特に渓流のフライ・フィッシング)、薪ストーブ