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Faculty Members
Faculty of Biotechnology / Department of Bioscience Associate professor

Itoh Takafumi

Profile Research Interest Selected Publications Social Activities Educational Activies Additional Information

Profile

Date of Birth1975.12.15
AffiliationFaculty of Biotechnology / Department of Bioscience
TitleAssociate professor
RoomFaculty of Biotechnology318 (Extension 3318)
E-mailito-t at fpu.ac.jp ( at = @ )
EducationPh.D. Kyoto Univ. 2006; M. Agr. Kyoto Univ. 2001; B.Agr. Kyoto Univ. 1999
Academic Societies

Japan Society for Bioscience, Biotechnology, and Agrochemistry; The Japanease Biochemical Society; The Society for Biotechnology, Japan; The Biophysical Society of Japan

Career

Associate Professor, Department of Bioscience, Fukui Prefectural University (2011 - present)
Assistant Professor, Department of Bioscience, Fukui Prefectural University (2010 - 2011)
Researcher, Graduate School of Agriculture, Kyoto University (2003 - 2010)
Researcher, Sumitomo Chemical Co., Ltd. (2001 - 2003)

Research Interest

Research FieldStructural biology; Enzyme chemistry; protein engineering; applied microbiology
Research Summary

Our group use X-ray crystallography, biochemistry, and molecular biology to investigate the enzyme structure-function relationships. The current main research focuses on polysaccharide glycoside hydrolases and carbohydrate binding proteins. Our goal is to provide new functional biomaterials from abundant natural resources such as chitin by using these macromolecules.

KeywordsEnzyme & protein chemistry; structural biology

Selected Publications

Research articles
1. Itoh, T., Mikami, B., Maru, I., Ohta, Y., Hashimoto, W. and Murata, K.: Crystal structure of N-acyl-D-glucosamine 2-epimerase from porcine kidney at 2.0 A resolution.
J. Mol. Biol. 303: 733-744, 2000.
2. Itoh, T., Akao, S., Hashimoto, W., Mikami, B. and Murata, K.: Crystal structure of unsaturated glucuronyl hydrolase, responsible for the degradation of glycosaminoglycan, from Bacillus sp. GL1 at 1.8 A resolution.
J. Biol. Chem. 279: 31804-31812, 2004.
3. Itoh, T., Ochiai, A., Mikami, B., Hashimoto, W. and Murata, K.: A novel glycoside hydrolase family 105: the structure of family 105 unsaturated rhamnogalacturonyl hydrolase complexed with a disaccharide in comparison with family 88 enzyme complexed with the disaccharide.
J. Mol. Biol. 360: 573-585, 2006.
4. Itoh, T., Hashimoto, W., Mikami, B. and Murata, K.: Crystal structure of unsaturated glucuronyl hydrolase complexed with substrate: molecular insights into its catalytic reaction mechanism.
J. Biol. Chem. 281: 29807-29816, 2006.
5. Itoh, T., Hashimoto, W., Mikami, B. and Murata, K.: Substrate recognition of unsaturated glucuronyl hydrolase from Bacillus sp. GL1.
Biochem. Biophys. Res. Commun. 344: 253-262, 2006.
6. Itoh, T., Ochiai, A., Mikami, B., Hashimoto, W. and Murata, K.: Structure of unsaturated rhamnogalacturonyl hydrolase complexed with substrate.
Biochem. Biophys. Res. Commun. 347: 1021-1029, 2006.
7. Itoh, T., Garcia, R. N., Adachi, M., Maruyama, Y., Tecson-Mendoza, E. M., Mikami, B. and Utsumi, S.: Structure of 8Sα globulin, the major seed storage protein of mungbean.
Acta Crystallograph. Sect. D Biol. Crystallogr. 62: 824-832, 2006.
8. Malle, D., Itoh, T., Hashimoto, W., Murata, K., Utsumi, S. and Mikami, B.: Overexpression, purification and preliminary X-ray analysis of pullulanase from Bacillus subtilis strain 168.
Acta Crystallograph. Sect. F Struct. Biol. Cryst. Commun. 62: 381-384, 2006.
9. Ochiai, A., Yamasaki, M., Itoh, T., Mikami, B., Hashimoto, W. and Murata, K.: Crystallization and preliminary X-ray analysis of the rhamnogalacturonan lyase YesW from Bacillus subtilis strain 168, a member of polysaccharide lyase family 11.
Acta Crystallograph. Sect. F Struct. Biol. Cryst. Commun. 62: 438-440, 2006.
10. Ochiai, A., Itoh, T., Kawamata, A., Hashimoto, W. and Murata, K.: Plant cell wall degradation by saprophytic Bacillus subtilis: gene clusters responsible for rhamnogalacturonan depolymerization.
Appl. Environ. Microbiol. 73: 3803-3813, 2007.
11. Ochiai, A., Itoh, T., Maruyama, Y., Kawamata, A., Mikami, B., Hashimoto, W. and Murata, K.: A novel structural fold in polysaccharide lyases: Bacillus subtitis family 11 rhamnogalacturonan lyase YesW with an eight-braded-propeller.
J. Biol. Chem. 282: 37134-37145, 2007.
12. Takita, T., Aono, T., Sakurama, H., Itoh, T., Wada, T., Minoda, M., Yasukawa, K. and Inouye, K.: Effects of introducing negative charges into the molecular surface of thermolysin by site-directed mutagenesis on its activity and stability.
Biochim. Biophys. Acta. 1784: 481-488, 2008.
13. Itoh, T., Mikami, B., Hashimoto, W. and Murata, K.: Crystal structure of YihS in complex with D-mannose: structural annotation of Escherichia coli and Salmonella enterica yihS-encoded proteins to an aldose-ketose isomerase.
J. Mol. Biol. 377: 1443-1459, 2008.
14. Sakurama, H., Takita, T., Mikami, B., Itoh, T., Yasukawa, K. and Inouye, K.: Two crystal structures of lysyl-tRNA synthetase from Bacillus stearothermophilus in complex with lysyladenylate-like compounds: insights into the irreversible formation of the enzyme-bound adenylate of L-lysine hydroxamate.
J. Biochem. 145, 555-563, 2009.
15. Ochiai, A., Itoh, T., Mikami, B., Hashimoto, W. and Murata, K.: Structural determinants responsible for substrate recognition and mode of action in family 11 polysaccharide lyases.
J. Biol. Chem. 284: 10181-10189, 2009.
16. Hashimoto, W., Ochiai, A., Momma, K., Itoh, T., Mikami, B., Maruyama, Y. and Murata, K.: Crystal structure of the glycosidase family 73 peptidoglycan hydrolase FlgJ.
Biochem. Biophys. Res. Commun. 381: 16-21, 2009.
17. Hashimoto, W., Ochiai, A., He, J., Itoh, T., Mikami, B. and Murata, K.: Crystallization and preliminary crystallographic analysis of the cell-surface alginate-binding protein Algp7 isolated from Sphingomonas sp. A1.
Acta Crystallogr. Sect F Struct. Biol. Cryst. Commun. 65: 515-517, 2009.
18. Maruyama, Y., Nakamichi, Y., Itoh, T., Mikami, B., Hashimoto, W. and Murata, K.: Substrate specificity of streptococcal unsaturated glucuronyl hydrolases for sulfated glycosaminoglycan.
J. Biol. Chem. 284: 18059-18069, 2009.
19. Tandang-Silvas, M. R., Fukuda, T., Fukuda, C., Prak, K., Cabanos, C., Kimura, A., Itoh, T., Mikami, B., Utsumi, S. and Maruyama, N. Conservation and divergence on plant seed 11S globulins based on crystal structures.
Biochim. Biophys. Acta. 1804: 1432-1442, 2010.
20. Maruyama, Y., Ochiai, A., Itoh, T., Mikami, B., Hashimoto, W. and Murata, K.: Mutational studies of the peptidoglycan hydrolase FlgJ of Sphingomonas sp. strain A1.
J. Basic. Microbiol. 50: 311-317, 2010.
21. Torio, M. A. O, Itoh, T., Garcia, R. N., Maruyama, N., Utsumi, S. and Tecson-Mendoza, E. M.: Introduction of sulfhydryl groups and disulfide linkage to mungbean 8Sα globulin and effects on physicochemical and functional properties.
Food Res. Int. 45: 277-282, 2012.
22. Maruyama, Y., Itoh, T., Nishitani, Y., Mikami, B., Hashimoto, W. and Murata, K: Crystallization and preliminary X-ray analysis of alginate importer from Sphingomonas sp. A1.
Acta Crystallogr. Sect F Struct. Biol. Cryst. Commun. 68: 317-320, 2012.
23. Nishitani, Y., Maruyama, Y., Itoh, T., Mikami, B., Hashimoto, W. and Murata, K.: Recognition of Heteropolysaccharide Alginate by Periplasmic Solute-Binding Proteins of a Bacterial ABC Transporter.
Biochemistry 51: 3622-3633, 2012.
24. Nasuno, R., Hirano, Y., Itoh, T., Hakoshima, T., Hibi, T. and Takagi, H.: Structural and functional analysis of the yeast N-acetyltransferase Mpr1 involved in oxidative stress tolerance via proline metabolism.
Proc. Natl. Acad. Sci. USA. 110: 11821-11826, 2013.
25. Prak, K., Mikami, B., Itoh, T., Fukuda, T., Maruyama, N. and Utsumi, S.: Purification, crystallization and preliminary crystallographic analysis of the soybean glycinin A1bB2.
Acta Crystallogr. Sect F Struct. Biol. Cryst. Commun. 69: 937-941, 2013.
26. Itoh, T., Hibi, T., Fujii, Y., Sugimoto, I., Fujiwara, A., Suzuki, F., Iwasaki, Y., Kim, J-K., Taketo, A. and Kimoto, H.: Cooperative degradation of chitin by extracellular and cell surface-expressed chitinases from Paenibacillus sp. FPU-7.
Appl. Environ. Microbiol. 79: 7482-7490, 2013.
27. Itoh, T., Hibi, T., Sugimoto, I., Suzuki, F., Fujii, Y., Taketo, A. and Kimoto, H.: Crystallization and preliminary X-ray analysis of the catalytic domains of Paenibacillus sp. str. FPU-7 cell surface-expressed chitinase ChiW.
Acta Crystallogr. Sect F Struct. Biol. Cryst. Commun. 70, 350-353, 2014.
28. Itoh, T., Sugimoto, I., Hibi, T., Suzuki, F., Matsuo, K., Fujii, Y., Taketo, A. and Kimoto, H.: Overexpression, purification, and characterization of Paenibacillus cell surface-expressed chitinase ChiW with two catalytic domains.
Biosci. Biotechnol. Biochem. 78: 624-634, 2014.
29. Suyotha, W., Yano, S., Itoh, T., Fujimoto, H., Hibi, T., Tachiki, T. and Wakayama, M.: Characterization of α-1,3-glucanase isozyme from Paenibacillus glycanilyticus FH11 in a new subgroup of family 87 α-1,3-glucanase.
J. Biosci. Bioeng. 118: 378-385, 2014.
30. Hibi, T., Hayashi, Y., Fukada, H., Itoh, T., Nago, T. and Nishiya, Y.: Intersubunit salt bridges with a sulfate anion control subunit dissociation and thermal stabilization of Bacillus sp. TB-90 urate oxidase.
Biochemistry 53: 3879-3888, 2014.
31. Katano, H., Takakuwa, M., Itoh, T. and Hibi, T.: Colorimetric determination of fructose for the high-throughput microtiter plate assay of glucose isomerase.
Biosci. Biotechnol. Biochem. 79: 1057-1060, 2015.
32. Katano, H., Takakuwa, M., Itoh, T. and Hibi, T.: Microplate assay of α-glucosidase and its inhibitors based on the direct reduction of molybdosilicate by glucose.
Anal. Sci. 3:1291-1295, 2015.
33. Maruyama, Y., Itoh, T., Kaneko, A., Nishitani, Y., Mikami, B., Hashimoto, W. and Murata, K.: Structure of a bacterial ABC transporter involved in the import of an acidic polysaccharide alginate.
Structure 23: 1643-1654, 2015.
34. Hibi, T., Kume, A., Kawamura, A., Itoh, T., Fukada, H. and Nishiya, Y.: Hyperstabilization of tetrameric Bacillus sp. TB-90 urate oxidase by introducing disulfide bonds through structural plasticity.
Biochemistry 55: 724-732, 2016.

Book
1. Hashimoto, W., Maruyama, Y., Itoh, T., Mikami, B. and Murata, K.: Bacterial system for alginate uptake and degradation.
Alginates: Biology and Applications, Microbiology Monographs, 13, 73-94, 2009, Rehm, B. H. A. (Ed.), Springer, Berlin, Germany.

Reviews
1. Hashimoto, W., Yamasaki, M., Itoh, T., Momma, K., Mikami, B. and Murata, K.: Super-channel in bacteria: structural and functional aspects of a novel biosystem for the import and depolymerization of macromolecules.
J. Biosci. Bioeng. 98: 399-413, 2004.
2. Hashimoto, W., Itoh, T., Maruyama, Y., Mikami, B. and Murata, K.: Hydration of vinyl ether groups by unsaturated glycoside hydrolases and their role in bacterial pathogenesis.
Int. Microbiol. 10: 233-243, 2007.

Educational Activies

ClassesSeminar in Applied Biochemistry; Physical Biochemistry II; Elementary Laboratory Course in Chemistry; Laboratory Course in Functional and Analytical Food Science; Laboratory Course in Physical Biochemistry

Additional Information

The Japan Bioscience, Biotechnology and Agrochemistry Society Award for the Encuragement of Young Scientists, 2015.

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