English
教員情報
生物資源学部 / 生物資源学科 准教授

いとう たかふみ伊藤 貴文

プロフィール 研究活動 主な著書・論文 主な社会・地域活動 教育活動 その他

プロフィール

生年月日1975年12月15日
所属生物資源学部 / 生物資源学科
生物資源学研究科
職名准教授
研究室生物資源学部棟318号室 (内線3318)
E-mailito-t (末尾に「@fpu.ac.jp」をつけてください)
最終学歴京都大学 農学部卒, 京都大学大学院 農学研究科 修士課程修了
所属学会

日本農芸化学会; 日本生化学会; 日本生物工学会; 日本生物物理学会

職歴

2001 住友化学工業(株) 研究員
2003 京都大学大学院 農学研究科 産学官連携研究員
2007 京都大学大学院 農学研究科 COE研究員
2007 京都大学大学院 農学研究科 産学官連携研究員
2010 福井県立大学 助教
2011 福井県立大学 講師
2014 現職

研究活動

専門酵素化学, タンパク質工学, 構造生物学, 応用微生物学
研究活動の紹介

タンパク質・酵素の構造と機能の相関を解明するために、タンパク質工学、構造生物学、進化工学、生物物理学、分子生物学などあらゆるアプローチから研究を行っています。そして、タンパク質・酵素の立体構造構築原理を解明することにより、新規なタンパク質や酵素を論理的に創出する基礎を確立し、科学や産業の発展に貢献したいです。
下記のテーマについて研究を行っています。
(1) 糖質代謝関連酵素に関する研究
(2) 細菌ABCトランスポーターに関する研究
(3) 植物種子貯蔵タンパク質に関する研究

キーワード酵素, タンパク質, X線結晶構造解析

主な著書・論文

【原 著 論 文】
1. Itoh, T., Mikami, B., Maru, I., Ohta, Y., Hashimoto, W. and Murata, K.: Crystal structure of N-acyl-D-glucosamine 2-epimerase from porcine kidney at 2.0 A resolution.
J. Mol. Biol. 303: 733-744, 2000.
2. Itoh, T., Akao, S., Hashimoto, W., Mikami, B. and Murata, K.: Crystal structure of unsaturated glucuronyl hydrolase, responsible for the degradation of glycosaminoglycan, from Bacillus sp. GL1 at 1.8 A resolution.
J. Biol. Chem. 279: 31804-31812, 2004.
3. Itoh, T., Ochiai, A., Mikami, B., Hashimoto, W. and Murata, K.: A novel glycoside hydrolase family 105: the structure of family 105 unsaturated rhamnogalacturonyl hydrolase complexed with a disaccharide in comparison with family 88 enzyme complexed with the disaccharide.
J. Mol. Biol. 360: 573-585, 2006.
4. Itoh, T., Hashimoto, W., Mikami, B. and Murata, K.: Crystal structure of unsaturated glucuronyl hydrolase complexed with substrate: molecular insights into its catalytic reaction mechanism.
J. Biol. Chem. 281: 29807-29816, 2006.
5. Itoh, T., Hashimoto, W., Mikami, B. and Murata, K.: Substrate recognition of unsaturated glucuronyl hydrolase from Bacillus sp. GL1.
Biochem. Biophys. Res. Commun. 344: 253-262, 2006.
6. Itoh, T., Ochiai, A., Mikami, B., Hashimoto, W. and Murata, K.: Structure of unsaturated rhamnogalacturonyl hydrolase complexed with substrate.
Biochem. Biophys. Res. Commun. 347: 1021-1029, 2006.
7. Itoh, T., Garcia, R. N., Adachi, M., Maruyama, Y., Tecson-Mendoza, E. M., Mikami, B. and Utsumi, S.: Structure of 8Sα globulin, the major seed storage protein of mungbean.
Acta Crystallograph. Sect. D Biol. Crystallogr. 62: 824-832, 2006.
8. Malle, D., Itoh, T., Hashimoto, W., Murata, K., Utsumi, S. and Mikami, B.: Overexpression, purification and preliminary X-ray analysis of pullulanase from Bacillus subtilis strain 168.
Acta Crystallograph. Sect. F Struct. Biol. Cryst. Commun. 62: 381-384, 2006.
9. Ochiai, A., Yamasaki, M., Itoh, T., Mikami, B., Hashimoto, W. and Murata, K.: Crystallization and preliminary X-ray analysis of the rhamnogalacturonan lyase YesW from Bacillus subtilis strain 168, a member of polysaccharide lyase family 11.
Acta Crystallograph. Sect. F Struct. Biol. Cryst. Commun. 62: 438-440, 2006.
10. Ochiai, A., Itoh, T., Kawamata, A., Hashimoto, W. and Murata, K.: Plant cell wall degradation by saprophytic Bacillus subtilis: gene clusters responsible for rhamnogalacturonan depolymerization.
Appl. Environ. Microbiol. 73: 3803-3813, 2007.
11. Ochiai, A., Itoh, T., Maruyama, Y., Kawamata, A., Mikami, B., Hashimoto, W. and Murata, K.: A novel structural fold in polysaccharide lyases: Bacillus subtitis family 11 rhamnogalacturonan lyase YesW with an eight-braded-propeller.
J. Biol. Chem. 282: 37134-37145, 2007.
12. Takita, T., Aono, T., Sakurama, H., Itoh, T., Wada, T., Minoda, M., Yasukawa, K. and Inouye, K.: Effects of introducing negative charges into the molecular surface of thermolysin by site-directed mutagenesis on its activity and stability.
Biochim. Biophys. Acta. 1784: 481-488, 2008.
13. Itoh, T., Mikami, B., Hashimoto, W. and Murata, K.: Crystal structure of YihS in complex with D-mannose: structural annotation of Escherichia coli and Salmonella enterica yihS-encoded proteins to an aldose-ketose isomerase.
J. Mol. Biol. 377: 1443-1459, 2008.
14. Sakurama, H., Takita, T., Mikami, B., Itoh, T., Yasukawa, K. and Inouye, K.: Two crystal structures of lysyl-tRNA synthetase from Bacillus stearothermophilus in complex with lysyladenylate-like compounds: insights into the irreversible formation of the enzyme-bound adenylate of L-lysine hydroxamate.
J. Biochem. 145, 555-563, 2009.
15. Ochiai, A., Itoh, T., Mikami, B., Hashimoto, W. and Murata, K.: Structural determinants responsible for substrate recognition and mode of action in family 11 polysaccharide lyases.
J. Biol. Chem. 284: 10181-10189, 2009.
16. Hashimoto, W., Ochiai, A., Momma, K., Itoh, T., Mikami, B., Maruyama, Y. and Murata, K.: Crystal structure of the glycosidase family 73 peptidoglycan hydrolase FlgJ.
Biochem. Biophys. Res. Commun. 381: 16-21, 2009.
17. Hashimoto, W., Ochiai, A., He, J., Itoh, T., Mikami, B. and Murata, K.: Crystallization and preliminary crystallographic analysis of the cell-surface alginate-binding protein Algp7 isolated from Sphingomonas sp. A1.
Acta Crystallogr. Sect F Struct. Biol. Cryst. Commun. 65: 515-517, 2009.
18. Maruyama, Y., Nakamichi, Y., Itoh, T., Mikami, B., Hashimoto, W. and Murata, K.: Substrate specificity of streptococcal unsaturated glucuronyl hydrolases for sulfated glycosaminoglycan.
J. Biol. Chem. 284: 18059-18069, 2009.
19. Tandang-Silvas, M. R., Fukuda, T., Fukuda, C., Prak, K., Cabanos, C., Kimura, A., Itoh, T., Mikami, B., Utsumi, S. and Maruyama, N. Conservation and divergence on plant seed 11S globulins based on crystal structures.
Biochim. Biophys. Acta. 1804: 1432-1442, 2010.
20. Maruyama, Y., Ochiai, A., Itoh, T., Mikami, B., Hashimoto, W. and Murata, K.: Mutational studies of the peptidoglycan hydrolase FlgJ of Sphingomonas sp. strain A1.
J. Basic. Microbiol. 50: 311-317, 2010.
21. Torio, M. A. O, Itoh, T., Garcia, R. N., Maruyama, N., Utsumi, S. and Tecson-Mendoza, E. M.: Introduction of sulfhydryl groups and disulfide linkage to mungbean 8Sα globulin and effects on physicochemical and functional properties.
Food Res. Int. 45: 277-282, 2012.
22. Maruyama, Y., Itoh, T., Nishitani, Y., Mikami, B., Hashimoto, W. and Murata, K: Crystallization and preliminary X-ray analysis of alginate importer from Sphingomonas sp. A1.
Acta Crystallogr. Sect F Struct. Biol. Cryst. Commun. 68: 317-320, 2012.
23. Nishitani, Y., Maruyama, Y., Itoh, T., Mikami, B., Hashimoto, W. and Murata, K.: Recognition of Heteropolysaccharide Alginate by Periplasmic Solute-Binding Proteins of a Bacterial ABC Transporter.
Biochemistry 51: 3622-3633, 2012.
24. Nasuno, R., Hirano, Y., Itoh, T., Hakoshima, T., Hibi, T. and Takagi, H.: Structural and functional analysis of the yeast N-acetyltransferase Mpr1 involved in oxidative stress tolerance via proline metabolism.
Proc. Natl. Acad. Sci. USA. 110: 11821-11826, 2013.
25. Prak, K., Mikami, B., Itoh, T., Fukuda, T., Maruyama, N. and Utsumi, S.: Purification, crystallization and preliminary crystallographic analysis of the soybean glycinin A1bB2.
Acta Crystallogr. Sect F Struct. Biol. Cryst. Commun. 69: 937-941, 2013.
26. Itoh, T., Hibi, T., Fujii, Y., Sugimoto, I., Fujiwara, A., Suzuki, F., Iwasaki, Y., Kim, J-K., Taketo, A. and Kimoto, H.: Cooperative degradation of chitin by extracellular and cell surface-expressed chitinases from Paenibacillus sp. FPU-7.
Appl. Environ. Microbiol. 79: 7482-7490, 2013.
27. Itoh, T., Hibi, T., Sugimoto, I., Suzuki, F., Fujii, Y., Taketo, A. and Kimoto, H.: Crystallization and preliminary X-ray analysis of the catalytic domains of Paenibacillus sp. str. FPU-7 cell surface-expressed chitinase ChiW.
Acta Crystallogr. Sect F Struct. Biol. Cryst. Commun. 70, 350-353, 2014.
28. Itoh, T., Sugimoto, I., Hibi, T., Suzuki, F., Matsuo, K., Fujii, Y., Taketo, A. and Kimoto, H.: Overexpression, purification, and characterization of Paenibacillus cell surface-expressed chitinase ChiW with two catalytic domains.
Biosci. Biotechnol. Biochem. 78: 624-634, 2014.
29. Suyotha, W., Yano, S., Itoh, T., Fujimoto, H., Hibi, T., Tachiki, T. and Wakayama, M.: Characterization of α-1,3-glucanase isozyme from Paenibacillus glycanilyticus FH11 in a new subgroup of family 87 α-1,3-glucanase.
J. Biosci. Bioeng. 118: 378-385, 2014.
30. Hibi, T., Hayashi, Y., Fukada, H., Itoh, T., Nago, T. and Nishiya, Y.: Intersubunit salt bridges with a sulfate anion control subunit dissociation and thermal stabilization of Bacillus sp. TB-90 urate oxidase.
Biochemistry 53: 3879-3888, 2014.
31. Katano, H., Takakuwa, M., Itoh, T. and Hibi, T.: Colorimetric determination of fructose for the high-throughput microtiter plate assay of glucose isomerase.
Biosci. Biotechnol. Biochem. 79: 1057-1060, 2015.
32. Katano, H., Takakuwa, M., Itoh, T. and Hibi, T.: Microplate assay of α-glucosidase and its inhibitors based on the direct reduction of molybdosilicate by glucose.
Anal. Sci. 3:1291-1295, 2015.
33. Maruyama, Y., Itoh, T., Kaneko, A., Nishitani, Y., Mikami, B., Hashimoto, W. and Murata, K.: Structure of a bacterial ABC transporter involved in the import of an acidic polysaccharide alginate.
Structure 23: 1643-1654, 2015.
34. Hibi, T., Kume, A., Kawamura, A., Itoh, T., Fukada, H. and Nishiya, Y.: Hyperstabilization of tetrameric Bacillus sp. TB-90 urate oxidase by introducing disulfide bonds through structural plasticity.
Biochemistry 55: 724-732, 2016.

【著 書】
Bacterial system for alginate uptake and degradation. Alginates: Biology and Applications, Microbiology Monographs, 13, 73-94, 2009, Rehm, B. H. A. (Ed.), Springer, Berlin, Germany. Hashimoto, W., Maruyama, Y., Itoh, T., Mikami, B. and Murata, K.

【総 説】
1. Hydration of vinyl ether groups by unsaturated glycoside hydrolases and their role in bacterial pathogenesis. Hashimoto, W., Itoh, T., Maruyama, Y., Mikami, B. and Murata, K. Int. Microbiol. 10: 233-243, 2007.
2. Super-channel in bacteria: structural and functional aspects of a novel biosystem for the import and depolymerization of macromolecules. Hashimoto, W., Yamasaki, M., Itoh, T., Momma, K., Mikami, B. and Murata, K. J. Biosci. Bioeng. 98: 399-413, 2004.
3. ブタ腎臓由来N-アシル-D-グルコサミン 2-エピメラーゼの構造、機能及び工業的利用に関する研究. 丸勇史, 大西淳, 太田泰弘, 伊藤貴文, 橋本渉, 三上文三, 村田幸作. 応用微生物学研究 2: 12-21, 2004.
4. Paenibacillus属細菌のキチン分解機構. 木元久, 伊藤貴文, 日び隆雄, 藤井豊, 草桶秀雄. 応用糖質化学 4: 113-120, 2014.

教育活動

担当科目応用生化学演習、生物物理化学II、化学実験、食品生化学実験、生物物理化学実験
相談・講演・共同研究等に応じられるテーマなど

糖質代謝関連酵素の利用 産業用酵素の高機能化 タンパク質のX線結晶構造解析

その他

学位:農学博士(京都大学)
受賞:農芸化学奨励賞 (日本農芸化学会, 2015年)

↑