Search
Kurokawa Yoichi
- Profile
- Research Interest
- Selected Publications
- Community Engagement
- Educational Activities
- Additional Information
Profile
Research Interest
| Research Field | Biochemistry, Enzyme chemistry, Protein science |
|---|---|
| Research Summary | Investigation of molecular basis for abonormal protein formation, and its application Heterologous production of human protein causative of a nervous disease, and exploration of chemicals that can prevent abnormal poduct of the protein |
| Keywords | stress response, abnormal protein, oxidative stress, antioxidant |
Selected Publications
Overexpression of Bacterial Protein Disulfide Isomerase (DsbC) and Its Modulator Protein (DsbD) Markedly Enhances Periplasmic Production of Human Nerve Growth Factor in Escherichia coli.
Journal of Biological Chemistry 276. 14393-14399 (2001)
Combination of Dsb coexpression and an addition of sorbitol markedly enhanced soluble expression of single-chain Fv in Escherichia coli.Biotechnol Bioeng. 91(4):418-24. (2005)
The functional effect of Gly209 and Ile213 substitutions on lysozyme activity of family 19 chitinase encoded by cyanophage Ma-LMM01 Fisheries Science 77 (4):665-70. (2011)
Community Engagement
Japan Society for Bioscience, Biotechnology, and Agrochemistry, The Japanese Biochemical Society, Protein Science Socity of Japan, The VItamin Society of Japan
Educational Activities
| Classes | Chemistry II, Biopolymer Chemistry, Experiments of Chemistry, Laboratory Course in Physical Biochemistry, Laboratory Course in Functional and Analytical Food Science |
|---|---|
| Office Hour | |
| Available Topics | Efficient expression system for useful heterologous protein How protein abnormalizes, and becomes a causative of a disease Oxidation of protein under oxidative stress Molecular aspects of function of chemicals of food-origin, especially vitamins and polyphenols |
Additional Information
I am interested to know how proteins, functional biomolecules, are abnormalized and the charecteristics of the abnormalized proteins, in order to contribute to promote human health. Molecular aspects on the interaction of protein and chemicals are also of my great interest.