Hibi Takao



Affiliation Faculty of Biotechnology / Department of Bioscience
Title Professor
Room Faculty of Biotechnology 314 (Extension3314)
E-mail hibi ( at = @ )
Education Ph. D. (The Graduate School of Agriculture, Kyoto University, Division of Biochemistry)
Academic Societies Japan Society for Bioscience, Biotechnology, and Agrochemistry,
Protein Science Society of Japan,
The Crystallographic Society of Japan,
The Japanese Biochemical Society,
The vitamin society of Japan,
The Polarographic Society of Japan
Career 1994-1996 Researcher, Kao Co. Ltd., Food Science laboratory
1996-2004 Lecturer, Dept. of Bioscience, Fukui Prefectural Univ.
2005-2011 Associate Professor, Dept. of Bioscience, Fukui Prefectural Univ.
2011-present Professor, Dept. of Bioscience, Fukui Prefectural University

Research Interest

Research Field Structural Biology, Protein Engineering
Research Summary We use X-ray crystallography and many other techniques (1) to understand the relationships between the structural plasticity of enzymes and its catalytic function and (2) to exploit this knowledge for the design and discovery of new enzymes for industrial applications: enzyme analysis, production of valuable fine chemicals, etc.
Keywords Enzyme Bioengineering Protein structure

Selected Publications

T. Hibi, A. Kume, A. Kawamura, T. Itoh, H. Fukada, and Y. Nishiya, "Hyperstabilization of Tetrameric Bacillus sp. TB-90 Urate Oxidase by Introducing Disulfide Bonds through Structural Plasticity", Biochemistry, (2016)
DOI: 10.1021/acs.biochem.5b01119

H. Katano, M. Takakuwa, T. Itoh & T. Hibi, "Microplate Assay of α-Glucosidase and its Inhibitors Based on the Direct Reduction of Molybdosilicate by Glucose", Anal. Sci., 31(12): 1291-1295 (2015).

Hajime Katano, Masahiro Takakuwa, Takafumi Itoh & Takao Hibi, "Colorimetric determination of fructose for the high-throughput microtiter plate assay of glucose isomerase", Biochem. Biotech. Biosci., 79(7): 1057-1060 (2015). DOI:10.1080/09168451.2015.1010477

Takao Hibi, Yuta Hayashi, Harumi Fukada, Takafumi Itoh, Tomohiro Nago, and Yoshiaki Nishiya, "Intersubunit Salt Bridges with a Sulfate Anion Control Subunit Dissociation and Thermal Stabilization of Bacillus sp. TB-90 Urate Oxidase", Biochemistry, 53 (24): 3879-3888 (2014).

Takafumi Itoh, Takao Hibi, Fumiko Suzuki, Ikumi Sugimoto, Fumiko Suzuki, Yutaka Fujii, Akira Taketo, and Hisashi Kimoto, "Crystallization and preliminary X-ray analysis of the catalytic domains of Paenibacillus sp. strain FPU-7 cell-surface-expressed chitinase ChiW", Acta Crystallogr F Struct Biol Commun., 70(3): 350-3 (2014).

Takafumi Itoh, Ikumi Sugimoto, Takao Hibi, Fumiko Suzuki, Koichi Matsuo,Yutaka Fujii, Akira Taketo, and Hisashi Kimoto, "Overexpression, Purification, and Characterization of Paenibacillus Cell Surface-Expressed Chitinase ChiW with Two Catalytic Domains", Biochem. Biotech. Biosci., 78 (4): 624-634 (2014)

Ryo Nasuno, Yoshinori Hirano, Takafumi Itoh, Toshio Hakoshima, Takao Hibi and Hiroshi Takagi “Structural and functional analysis of the yeast N-acetyltransferase Mpr1 involved in oxidative stress tolerance via proline metabolism”, Proc. Natl. Sci. Acad. USA, 110(29), 11821–11826 (2013).

Takafumi Itoh, Takao Hibi, Yutaka Fujii, Ikumi Sugimoto, Akihiro Fujiwara, Fumiko Suzuki, Yukimoto Iwasaki, Jin-Kyung Kim, Akira Taketo, and Hisashi Kimoto, “Cooperative degradation of chitin by extracellular and cell surface-expressed chitinases from Paenibacillus sp. FPU-7”, Applied and Environmental Microbiology (2013), 79(23) 7482-7490.

Takafumi Itoh, Ikumi Sugimoto, Takao Hibi, Fumiko Suzuki, Koichi Matsuo,Yutaka Fujii, Akira Taketo, and Hisashi Kimoto “Overexpression, Purification, and Characterization of Paenibacillus Cell Surface-Expressed Chitinase ChiW with Two Catalytic Domains”, Biochem. Biotech. Biosci. (2013)

Shintaro Kubota, Osamu Shirai, Takao Hibi, Yuzuru Tozawa and Kenji Kano “Effect of Counter Ions on the Transport Current Across Membranes Containing KAT1 Potassium Channel”, Anal. Sci., 29, 161-163, (2013).

Takafumi Itoh, Takao Hibi, Yuta Hayashi, Ikumi Sugimoto, Fumiko Suzuki, Yutaka Fujii, and Hisashi Kimoto “Crystal Structure of Paenibacillus Chitinase ChiW catalytic domains complexed with pentasaccharide.”, SPring-8 activity report, 2012B, (2013).

Mai Hoa BT, Hibi T, Nasuno R, Matsuo G, Sasano Y, Takagi H., “Production of N-acetyl cis-4-hydroxy-l-proline by the yeast N-acetyltransferase Mpr1.”, J Biosci Bioeng. (2012).

T. Hibi, H. Yamamoto, G. Nakamura. and H. Takagi, “Crystallization and preliminary crystallographic analysis on N-acetyltransferase Mpr1 from Saccharomyces cerevisiae.” Acta Crystallogr. F65, 169-172 (2009).

T. Hibi, S. Aoki, K. Oda, S. Munemasa, S. Ozaki, O. Shirai, Y. Murata, N. Uozumi, “Purification of the functional plant membrane channel KAT1.” Biochemical and Biophysical Research Communications, 374(3), 465-469 (2008).

Hibi, T., Nii, H., Nakatsu, T., Kimura, A., Kato, H., Hiratake, J., and Oda, J. Crystal structure of γ -glutamylcysteine synthetase: Insights into the mechanism of catalysis by a key enzyme for glutathione homeostasis. Proceedings of the National Academy of Sciences of the United States of America (2004), 101(42), 15052-15057.

T. Hibi, T. Nishioka, H. Kato, K. Tanizawa, T. Fukui, Y. Katsube, J. Oda, Structure of the multifunctional loops in the nonclassical ATP-binding fold of glutathione synthetase. Nature Structural Biology (1996), 3(1), 16-18

Community Engagement

Educational Activities

Classes Organic Chemistry, Bio-Physicalchemistry, Structural biology
Office Hour
Available Topics

Additional Information